ctni primary antibody 1ab stock solution  (HyTest)

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 1. Gold secondary antibody was employed for cTnI detection, as shown in the plot: (A) entire spectrum absorption. The linearity for the different concentrations are all close to 0, indicating the wavelength and intensity do not have a linear correlation. While R2 is larger than 0.95, indicating all spectra are good fits in the quadratic curve; (B) absorption following blank-plate background correction, and (C) red shift vs log cTnI concentration. Indicate *c, the error bar decreased as cTnI concentration increased, indicating that studying an antibody’s dilution ratio can enhance resolution.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques: Concentration Assay

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 4. Secondary antibody dilution ratio with (A) different ratios, (B) 145× dilution, and (C) 1562.5× dilution. * Denotes that the reference wells contained 0 to 8 ng/mL standard cTnI protein; ** denotes that the sample contained human serum spiked with standard cTnI protein at three concentrations.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques:

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 3. cTnI detection model. (A) Standard curve calibrated at 450 nm absorbance. The arrow indicates the area used to increase resolution and also indicates the area of human cTnI with MI, which can vary and is highly dependent on the individual; (B) color change can be seen by the naked eye; color gradually changes in response to the cTnI concentration increased from 0.064 to 8.00 ng/mL.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques: Concentration Assay

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 6. Schematic illustrating the pricking of blood at the clinic or home for MI self-diagnosis. (A) Pricking a finger and adding one drop of blood into one sample well every 30 min; * indicates the reference wells. (B) Adding the reagents. (C) Self-diagnosing MI based on the yellow pattern, where the yellow color denotes a positive diagnosis. After comparing samples to the reference well and multiplying the converting factor, the doctor or patient can determine the cTnI protein concentration at each time point. Conversion factor 15 ± 7.14 for human serum and 1.84 ± 0.76 for 10× dilute human serum. For pricking point-of-care, 1.84 ± 0.76 can be an approximate conversion factor.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques: Biomarker Discovery, Protein Concentration

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 5. Schematic illustrating the naked-eye cTnI detection device in (A) sample wells; (B) reference wells; (C) blood loading on the sample well, with the red arrow representing blood flow; (D) PBS loading on the reference well, with the black arrow denoting PBS flow; (E,F) secondary antibody loading and chromophore acting. Different items indicate antibody (red Y-shape), BSA (blue ball), cTnI protein (gray), HRP (blue pistachio), and TMB chromophore (black ball). (A,C,E) represent sample wells, while (B,D,F) represent reference wells. The difference between sample and reference wells is that reference wells in the first step were already coated with standard cTnI protein, serving as calibration wells.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques:

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 8. Naked-eye cTnI detection device under a human serum matrix effect (A) spiked or (B) nonspiked with cTnI protein. The 10× diluted human serum (C) spiked or (D) nonspiked with cTnI protein. A spiked sample represents a patient sample (black arrow), while a nonspiked sample denotes a healthy sample. Black arrows represent 0, 0.5, and 1 h time points (from right to left) after the patient’s first blood- pricking. Each time point blood-prick once. * indicates 0, 0.064, 0.32, 1.60, and 8.00 ng/mL of cTnI protein as the reference wells from right to left and are the same in these four well strips. This device can distinguish between an MI and a healthy patient.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques:

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 7. Naked-eye cTnI detection device was tested and passed 7 months of life-shelf absorbance detectable data with (A) standard and (B) sample at day 1 and 7 months later. In addition, (C) device comprises three rows in a portable, disposable bag and can be assembled onto a 96-well frame. (D) Strips are sealed in an airtight package and stored at 4 °C after the fabrication. 7-month data shows color decreasing both at the sample and standard, but with the standard curve fitting, showing that the result is repetitive and readable.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques:

Journal: ACS pharmacology & translational science

Article Title: Rapid-Test Kit for Cardiac Troponin I: A Reliable Enzyme-Linked-Immuno-Substrate-Assay-Based Biosensor for Daily-Use Naked-Eye Detection and Pharmacokinetic Studies for Myocardial Infarction in Cardiovascular Disease.

doi: 10.1021/acsptsci.4c00218

Figure Lengend Snippet: Figure 9. Standard curve of cTnI protein between the proposed test strip and a commercial ELISA plate. The R2 of the proposed test strip is 0.9645. The linearity is (R2)1/2 = 0.9821.

Article Snippet: The cTnI primary antibody (1Ab) stock solution is prepared as follows: 2 μg/mL monoclonal mouse anti-cTnI (HyTest 4T21 cm3, 25 kDa) is dissolved in phosphate-buffered saline (PBS), and a multichannel pipet was used to add 100 μL/well.

Techniques: Stripping Membranes, Enzyme-linked Immunosorbent Assay